Cryo-EM Sample Prep:Article Title: Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis
Article Snippet: .. Cryo-EM data collection, refinement, and validation statistics SARS-Cov2 SPIKE CHO SARS-Cov2 SPIKE HEK Data collection and processing Microscope TFS Titan Krios TFS Titan Krios Detector Gatan K3/GIF (20eV) Gatan K3/GIF (20eV) Magnification 81,000× 81,000× Voltage (kV) 300 300 Electron exposure (e–/Å2) 66.5 66.5 Defocus range (μm) 0.8–2.5 0.8–2.5 Pixel size (Å) 1.058 1.058 Symmetry imposed C3 C3 Initial particle images (no.) 75,582 99,154 Final particle images (no.) 54,395 54,066 Map resolution (Å) 3.22 3.44 FSC threshold 0.143 0.143 Initial model used (PDB code) 6VXX 6VXX Model resolution (Å) 3.22 3.44 FSC threshold 0.143 0.143 Model resolution range (Å) ∞−3.22 ∞–3.44 Map sharpening B-factor (Å2) −86 −95 Model composition Non-hydrogen atoms 24,615 23,694 Protein residues 3,051 2,916 Ligand molecules 57 63 B-factors (Å2) 120 100 R.M.S. deviations Bond lengths (Å) 0.012 0.010 Bond angles (°) 1.041 1.046 Validation MolProbity score (percentile) 2.69 2.71 Clashscore (percentile) 24.29 24.48 Poor rotamers (%) 3.04 4.81 Ramachandran plot Favored (%) 92.93 95.46 Allowed (%) 7.07 4.54 Appendix 1. ..
Article Title:
Article Snippet: .. Cryo-EM data collection, refinement, and validation statistics SARS-Cov2 SPIKE ExpiCHO-S SARS-Cov2 SPIKE Expi293F Data collection and processing Microscope TFS Titan Krios TFS Titan Krios Detector Gatan K3/GIF (20eV) Gatan K3/GIF (20eV) Magnification 81,000× 81,000× Voltage (kV) 300 300 Electron exposure (e–/Å2) 66.5 66.5 Defocus range (μm) 0.8–2.5 0.8–2.5 Pixel size (Å) 1.058 1.058 Symmetry imposed C3 C3 Initial particle images (no.) 75,582 99,154 Final particle images (no.) 54,395 54,066 Map resolution (Å) 3.22 3.44 FSC threshold 0.143 0.143 Initial model used (PDB code) 6VXX 6VXX Model resolution (Å) 3.22 3.44 FSC threshold 0.143 0.143 Model resolution range (Å) ∞−3.22 ∞–3.44 Map sharpening B-factor (Å2) −86 −95 Model composition Non-hydrogen atoms 24,615 23,694 Protein residues 3,051 2,916 Ligand molecules 57 63 B-factors (Å2) 120 100 R.M.S. deviations Bond lengths (Å) 0.012 0.010 Bond angles (°) 1.041 1.046 Validation MolProbity score (percentile) 2.69 2.71 Clashscore (percentile) 24.29 24.48 Poor rotamers (%) 3.04 4.81 Ramachandran plot S13 Appendix 1. ..
Biomarker Discovery:Article Title: Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis
Article Snippet: .. Cryo-EM data collection, refinement, and validation statistics SARS-Cov2 SPIKE CHO SARS-Cov2 SPIKE HEK Data collection and processing Microscope TFS Titan Krios TFS Titan Krios Detector Gatan K3/GIF (20eV) Gatan K3/GIF (20eV) Magnification 81,000× 81,000× Voltage (kV) 300 300 Electron exposure (e–/Å2) 66.5 66.5 Defocus range (μm) 0.8–2.5 0.8–2.5 Pixel size (Å) 1.058 1.058 Symmetry imposed C3 C3 Initial particle images (no.) 75,582 99,154 Final particle images (no.) 54,395 54,066 Map resolution (Å) 3.22 3.44 FSC threshold 0.143 0.143 Initial model used (PDB code) 6VXX 6VXX Model resolution (Å) 3.22 3.44 FSC threshold 0.143 0.143 Model resolution range (Å) ∞−3.22 ∞–3.44 Map sharpening B-factor (Å2) −86 −95 Model composition Non-hydrogen atoms 24,615 23,694 Protein residues 3,051 2,916 Ligand molecules 57 63 B-factors (Å2) 120 100 R.M.S. deviations Bond lengths (Å) 0.012 0.010 Bond angles (°) 1.041 1.046 Validation MolProbity score (percentile) 2.69 2.71 Clashscore (percentile) 24.29 24.48 Poor rotamers (%) 3.04 4.81 Ramachandran plot Favored (%) 92.93 95.46 Allowed (%) 7.07 4.54 Appendix 1. ..
Article Title:
Article Snippet: .. Cryo-EM data collection, refinement, and validation statistics SARS-Cov2 SPIKE ExpiCHO-S SARS-Cov2 SPIKE Expi293F Data collection and processing Microscope TFS Titan Krios TFS Titan Krios Detector Gatan K3/GIF (20eV) Gatan K3/GIF (20eV) Magnification 81,000× 81,000× Voltage (kV) 300 300 Electron exposure (e–/Å2) 66.5 66.5 Defocus range (μm) 0.8–2.5 0.8–2.5 Pixel size (Å) 1.058 1.058 Symmetry imposed C3 C3 Initial particle images (no.) 75,582 99,154 Final particle images (no.) 54,395 54,066 Map resolution (Å) 3.22 3.44 FSC threshold 0.143 0.143 Initial model used (PDB code) 6VXX 6VXX Model resolution (Å) 3.22 3.44 FSC threshold 0.143 0.143 Model resolution range (Å) ∞−3.22 ∞–3.44 Map sharpening B-factor (Å2) −86 −95 Model composition Non-hydrogen atoms 24,615 23,694 Protein residues 3,051 2,916 Ligand molecules 57 63 B-factors (Å2) 120 100 R.M.S. deviations Bond lengths (Å) 0.012 0.010 Bond angles (°) 1.041 1.046 Validation MolProbity score (percentile) 2.69 2.71 Clashscore (percentile) 24.29 24.48 Poor rotamers (%) 3.04 4.81 Ramachandran plot S13 Appendix 1. ..
Microscopy:Article Title: Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis
Article Snippet: .. Cryo-EM data collection, refinement, and validation statistics SARS-Cov2 SPIKE CHO SARS-Cov2 SPIKE HEK Data collection and processing Microscope TFS Titan Krios TFS Titan Krios Detector Gatan K3/GIF (20eV) Gatan K3/GIF (20eV) Magnification 81,000× 81,000× Voltage (kV) 300 300 Electron exposure (e–/Å2) 66.5 66.5 Defocus range (μm) 0.8–2.5 0.8–2.5 Pixel size (Å) 1.058 1.058 Symmetry imposed C3 C3 Initial particle images (no.) 75,582 99,154 Final particle images (no.) 54,395 54,066 Map resolution (Å) 3.22 3.44 FSC threshold 0.143 0.143 Initial model used (PDB code) 6VXX 6VXX Model resolution (Å) 3.22 3.44 FSC threshold 0.143 0.143 Model resolution range (Å) ∞−3.22 ∞–3.44 Map sharpening B-factor (Å2) −86 −95 Model composition Non-hydrogen atoms 24,615 23,694 Protein residues 3,051 2,916 Ligand molecules 57 63 B-factors (Å2) 120 100 R.M.S. deviations Bond lengths (Å) 0.012 0.010 Bond angles (°) 1.041 1.046 Validation MolProbity score (percentile) 2.69 2.71 Clashscore (percentile) 24.29 24.48 Poor rotamers (%) 3.04 4.81 Ramachandran plot Favored (%) 92.93 95.46 Allowed (%) 7.07 4.54 Appendix 1. ..
Article Title:
Article Snippet: .. Cryo-EM data collection, refinement, and validation statistics SARS-Cov2 SPIKE ExpiCHO-S SARS-Cov2 SPIKE Expi293F Data collection and processing Microscope TFS Titan Krios TFS Titan Krios Detector Gatan K3/GIF (20eV) Gatan K3/GIF (20eV) Magnification 81,000× 81,000× Voltage (kV) 300 300 Electron exposure (e–/Å2) 66.5 66.5 Defocus range (μm) 0.8–2.5 0.8–2.5 Pixel size (Å) 1.058 1.058 Symmetry imposed C3 C3 Initial particle images (no.) 75,582 99,154 Final particle images (no.) 54,395 54,066 Map resolution (Å) 3.22 3.44 FSC threshold 0.143 0.143 Initial model used (PDB code) 6VXX 6VXX Model resolution (Å) 3.22 3.44 FSC threshold 0.143 0.143 Model resolution range (Å) ∞−3.22 ∞–3.44 Map sharpening B-factor (Å2) −86 −95 Model composition Non-hydrogen atoms 24,615 23,694 Protein residues 3,051 2,916 Ligand molecules 57 63 B-factors (Å2) 120 100 R.M.S. deviations Bond lengths (Å) 0.012 0.010 Bond angles (°) 1.041 1.046 Validation MolProbity score (percentile) 2.69 2.71 Clashscore (percentile) 24.29 24.48 Poor rotamers (%) 3.04 4.81 Ramachandran plot S13 Appendix 1. ..
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